Enzymes can be used as catalytic agent in industrial and medical applications. Such enzymes are immobilised by binding them to a solid, insoluble matrix, which will not affect the enzyme stability or its catalytic activity. Beaded gels and cyanogens bromideactivated sepharose are commonly used for immobilisation of enzymes. The bound enzymes can be preserved for a long period without loss of activity.

Example: Glucose oxidase and peroxidase are immobilised and coated on a strip of paper and are used in the clinical laboratory for the detection of glucose in urine. The intensity of blue colour depends on the concentration of glucose. Hence the strip method is useful for semi-quantitative estimation of glucose in urine.