Conventional methods of germplasm storage follows seed storage at 10–20°C at reduced moisture in sealed containers. Germplasm deteriorates under such conditions in plants such as rubber, neem, cocoa, jackfruit, mango, and litchi. For these and for seedless plants like tapioca, sugarcane, and sweet banana, germplasm storage may be in the form of cryopreservation of in vitro cultures of shoot tips, roots, somatic embryos, tissue, and pollen. Germplasm storage of endangered plants, primitive cultivars, and wild relatives of crop plants is undertaken to avoid erosion of plant genetic resources. Through cryopreservation, such as in vitro gene banks shall ensure availability of valuable germplasm to breeders to develop new and improved varieties. Many categories of endangered plants and living fossils like Ginkgo, Cycads, Metasequoia, and Cyathea apart from several Orchid species and medicinal plants Rauwolfia, Dioscorea, and Aconitum have been successfully conserved by tissue-culture methods.