- It is useful in evaluating the affinity of the enzyme; for the substrate, the low Km value of an E indicates its high affinity for[S].
- The Km value also gives an idea regarding the type of inhibition of an E caused by an inhibitor (double-reciprocal treatments).
- Km value is a useful index during the purification of enzymes.
- It has got biomedical importance in the treatment of leukaemia.
- It is also a useful index in the kinetic studies.
- It is used in the assay of enzymes.
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