Chemical Methods

Saponification number

Definition: Saponification number is defined as the milligrams of KOH required to hydrolyse (saponify) one gram of fat or oil.

Example:Human fat:195 – 200
 Butter:230 – 240
 Coconut oil:250 – 260

Significance: The saponification number thus provides information on the average chain length of fatty acids in the fat. It varies inversely with the chain length of the fatty acids. The shorter the average chain length of the fatty acids, the higher is the saponification number.


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