Identification

As sucrose is a reducing sugar, it shows negative results to reducing tests. Sucrose is dextrorotatory (+66.5°) and its hydrolytic products are glucose and fructose. The rotation of fructose is laevorotatory (−92°), whereas that of glucose is dextrorotatory (+52.7°). The resulting product of hydrolysis shows laevorotation. This change from dextrorotation to laevorotation is known as inversion. Hence, sucrose is also called as invert sugar. The net result is laevorotatory, resulting in change from +ve to −ve.

ch2-ufig12

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