LB plot

V = Vmax[S]/Km(1 + I/Ki) + [S]
1/V = Km(1 + I/Ki) + [S]/Vmax[S]
1/V = Km(1 + I/Ki)/Vmax[S] + [S]/Vmax[S]
1/V = Km(1 + I/Ki)/Vmax.1/[S] + 1/Vmax (refer Table 6.8)

 

Table 6.8 LB Equation for Competitive Inhibition

Slope (m)Km(1 + I/Ki)
Y intercept1/Vmax
X intercept−1/Km(1 + I/Ki)
  1. It is an LB plot (double-reciprocal plot) obtained in the absence of I and with two different concentration of a competitive inhibitor.
  2. Competitive inhibitor yields a family of lines with a common intercept on the 1/Vo axis but with different slopes.
  3. Since the y intercept is 1/Vmax, this states that an infinity high concentration of [S] (1/[S] = 0). Vi is the same as in the absence of inhibitor.
  4. However the x intercept varies with inhibitor concentration and becomes a larger number in the presence of the competitive Inhibitor.
  5. Thus, a competitive inhibitor varies the apparent Km for the substrate.

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