Fibrinogen is the soluble constituent of plasma and when thrombin is added to it, fibrinogen is converted to fibrin. After separation from plasma by fractionation, the precipitate is collected by centrifugation, dissolved in citrate saline and freeze-dried. The air in the containers is displaced by nitrogen. The citrate prevents spontaneous clotting when the material is…
Human plasma protein fraction is a solution of some of the proteins from liquid plasma. It contains albumin and certain globulins that retain their solubility on heating. It is prepared by fractionating pooled citrated plasma. Figure 10.1 shows the ether fractionation of plasma. Figure 10.1 Ether Fractionation of Plasma A stabilizer such as sodium caprylate or acetyl tryptophan…
This is prepared in the same method as dried plasma except that the blood is collected into dry bottles and allowed to clot. The supernatant serum is separated after the clot has retracted. Storage and use are the same as for dried plasma.
Preparation: Dried plasma is usually prepared from time-expired citrated blood. The supernatant fluid is separated from the blood by centrifugation process under aseptic conditions. Batches of not more than 10 bottles are pooled, choosing the correct ratio of blood groups to neutralize powerful agglutinins. The pools are kept at 4°C–6°C. Samples from the batch are tested…
The product is obtained by removing the citrated plasma from the whole blood that has been allowed to stand or has been centrifuged to deposit the cells. The supernatant liquid is removed under aseptic conditions. The cells are matched with the recipient’s plasma and may then be mixed with matched cells from other bottles. The…
This is human blood that has been mixed with a suitable anticoagulant. The use of anticoagulant mixtures such as ACD (acid citrate dextrose) and CPD (citrate phosphate dextrose) extends the useful life of the red cells. If stored under proper conditions, the blood can be safely used within a period of 21 days after collection. To…
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