Quantitative estimation of tannins in plant tissue is generally not accurate as other phenolic substances may interfere with the non-specific chemical methods. Also it is not possible to extract completely condensed tannins. Repeated measurements are needed to make an accurate estimation. Chemical assay methods based on functional groups such as Folin-Denis procedure for total phenols…
Solution of tannic acid precipitates heavy metals, alkaloids, glycosides and gelatin. For the standard solution, weigh 100 mg gallic acid reference sample and dissolve it in 100 ml water. Apply both the test and standard solutions to a precoated silica gel plate and develop in a solvent system of chloroform: ethyl formate: formic acid (5:4:1).…
Tannic acid of pharmaceutical grade is commercially obtained from nut galls, which are grown as pathological outgrowths on the stems and branches of the oak tree due to the deposition of eggs of the gall wasp. Galls are obtained in three grades namely blue, green and white. Tannic acid is extracted from these powdered galls…
Due to its astringent or protein-precipitating property, tannic acid is used as an antidiarrhoeal and styptic. Formerly it was used in the treatment of burns. It is topically used in the treatment of bed sores, skin ulcerations etc. As an alkaloidal precipitant it is used in alkaloid poisoning. Industrial uses of tannic acid include chemical…
Source Tannic acid or gallotannin is a commercial prepared form of tannin, a collective term applied to a group of complex polyphenolic secondary metabolites. These are present in localized structures associated with different plant parts. Tannins are distributed in both Angiosperms and Gymnosperms and are more common in dicotyledons than monocotyledons. Some important sources of…
Berberine gives precipitation with most of the alkaloidal reagents. Some specific tests for identification of berberine are listed below: Estimation Estimation of berberine in traditional medicine formulations and conventional dosage forms may be undertaken by several spectrophotometric, chromatographic and other methods. A. Method I (Gravimetric method) B. Method II (Spectrophotometric assay)
In Berberis species (B. aristata, B. glauca, B. vulgaris, B. aquifolium etc.) berberine alkaloids are principally located in the cortical tissues and the bark of old roots has the highest concentration. Upper parts of the shoot system have a low concentration of alkaloids and in young leaves they are not detectable. Berberine content varies depending on…
Source This alkaloid belongs to a group of modified benzyl tetrahydroisoquinoline alkaloids. A quarternary ammonium salt from the protoberberine group of isoquinoline alkaloids, it is found in many members of the Berberidaceae (Berberis and Mahonia species), Ranunculaceae (Hydrastis and Coptis species) and other families such as Anonaceae, Menispermaceae, Papaveraceae and Rutaceae. Berberine is found in…
Hesperidin estimation is reported in literature based on spectrophotometric and HPLC methods, with the latter being the method of choice for its determination in orange juice, body fluids and in herbal dosage forms. A. Method I (Spectrophotometric assay) B. Method II (HPLC method)
Estimation Hesperidin estimation is reported in literature based on spectrophotometric and HPLC methods, with the latter being the method of choice for its determination in orange juice, body fluids and in herbal dosage forms. A. Method I (Spectrophotometric assay) B. Method II (HPLC method)