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  • BIOSYNTHESIS OF PYRIMIDINE NUCLEOTIDES

    August 8, 2024
    Nucleotide Metabolism

    The synthesis of pyrimidine is a much simpler process compared to that of purines. Aspartate, glutamine (amide group), and CO2 contribute the atoms in the formation of pyrimidine ring.

  • INHIBITORS OF PURINE SYNTHESIS

    August 8, 2024
    Nucleotide Metabolism

    Folic acid (THF) is essential for the synthesis of purine nucleotides (reactions 4 and 10)-Refer to Figure 11.2. Sulphonamides are the structural analogues of para aminobenzoic acid (PABA). These sulpha drugs can be used to inhibit the synthesis of folic acid by micro-organisms. This indirectly reduces the synthesis of purines and therefore the nucleic acids (DNA…

  • Ion-binding capacity

    August 8, 2024
    Amino Acids

    As proteins are amphoteric in nature, they can form salts with both cations and anions. Many ions form insoluble salts with proteins and therefore they are used as precipitating agents for protein. Example: Anions of some acids like phosphotungstic, trichloroacetic acid, picric acid, and so on form insoluble acids with proteins. Heavy metals are used for…

  • DEGRADATION OF PURINE NUCLEOTIDES

    August 8, 2024
    Nucleotide Metabolism

    The end product of purine metabolism in human is uric acid. The sequence of reactions in purine nucleotides degradation is given below: Figure 11.6 Degradation of Purine Nucleotides

  • Amphoteric nature

    August 8, 2024
    Amino Acids

    Proteins are amphoteric in nature; that is, they have both acidic and basic properties. In the electric field, their migration depends upon the net charge carried by the molecule. The net charge is influenced by the pH value. Each protein has a specific pI value at which it will not move, because at this point…

  • Colloidal nature

    August 8, 2024
    Amino Acids

    Proteins exhibit colloidal properties. They show considerable light-scattering effect in solution and hence result in visible turbidity (Tyndall effect).

  • CONVERSION OF RIBONUCLEOTIDES TO DEOXYRIBONUCLEOTIDES

    August 8, 2024
    Nucleotide Metabolism

    The synthesis of purine and pyrimidine deoxyribonucleotides occurs from ribonucleotides by a reduction of the C2 of ribose moiety as shown in Figure 11.5. This reaction is catalysed by a multi-subunit (two B1 and two B2 subunits) enzyme ribonucleotide reductase. The ribonucleotide reductase itself provides the hydrogen atoms needed for the reduction from its sulfhydryl groups. The reducing equivalents,…

  • Molecular weight

    August 8, 2024
    Amino Acids

    Proteins vary in their molecular weight, which, in turn, is dependent on the number of amino acid residues. Each amino acid on an average contributes to a molecular weight of about 110. Majority of protein polypeptides may be composed of 40 to 40,000 amino acids with a molecular weight ranging from 4,000 to 4, 40,000.…

  • REGULATION OF PURINE NUCLEOTIDE BIOSYNTHESIS

    August 8, 2024
    Nucleotide Metabolism

    The purine nucleotide synthesis is well coordinated to meet the cellular demands. The intracellular concentration of PRPP regulates purine synthesis to a large extent. This increase depends on the availability of ribose 5′-phosphate and the enzyme PRPP synthase. PRPP glutamyl amidotransferase is controlled by a feedback mechanism by purine nucleotides. That is, if AMP and…

  • PROPERTIES OF PROTEINS

    August 8, 2024
    Amino Acids

    Physical properties Colour and taste: Proteins are colourless and usually tasteless. These are homogenous and crystalline. Shape and size: Proteins range in shape from simple crystalloid spherical structure to long fibrillar structure.

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